Silica Effects on the Bone Mineralization

Evaluation of SILICIUM G5 on Bone Health

Fibroblasts, Osteoblasts and MG-63 Cell Line

Performed at the Technology Centre for Nutrition and Health Survey

 

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Several authors have emphasized the role of silicon in health, being the most prominent its effect on the integrity of the nails, hair and skin as well as in the synthesis of collagen and bone mineralization. It was observed that silica deficiency has adverse effects such as bone deformities, joint malfunction, reducing the mass of cartilage and rupture of the mineral balance in femur and vertebrae.

One of the most suitable ways to determine possible effects of a bioactive compound or supplement is the use of in vitro systems on certain cell types, thus avoiding hormonal or endocrine interactions. The model chosen for this study was the MG-63 cell-line, which has a fibroblast phenotype which can be differentiated and give place to different populations of mature cells, such as osteoblasts.

The aim of this study is to evaluate the effects of Loic Le Ribault's, G5, organic silica on the production of collagen in the undifferentiated (fibroblasts) MG-63 cell line and the effects on differentiation and mineralization of osteoblasts.

 


Results of our Research


Effects of SILICIUM G5 on collagen production in the short term

 

We evaluated the effects of a treatment with 200, 1000 and 2000 mg / liter of silicon on the production of collagen, indicating its induction 24 hours after initiation of treatment compared with untreated cells.


Effects of SILICIUM G5 on collagen production long term

 

The same process performed over 16 days, resulted in a net increase in collagen production showing a dose-response action. The ratio of collagenous and non-collagenous proteins results in a significant 12% increase for all doses tested, indicating that the three treatments induce collagen synthesis.

 

Effects of SILICIUM G5 on calcium deposits in osteoblasts during differentiation

 

We studied whether treatments with SILICIUM G5 affected the calcification of MG-63 osteoblasts. The results show an increase in calcium deposition following a dose-response action, being statistically significant compared to untreated cells, corresponding to a 40% increase in calcification with a dosage of 2000 mg / liter

 

Effects of SILICIUM G5 on collagen synthesis in osteoblast during process of differentiation

 

The results show that treatment with the concentrated 2000 mg / liter product increases collagen levels in differentiated osteoblasts.

 

G5 SILICIUM effects on osteoblast differentiation

 

With the 2000 mg / liter product a significant increase, inducing the activity of alkaline phosphatase was observed.

 

Discussion

 

The results of the study show that collagen synthesis is enhanced by treatment with Loïc Le Ribault's, G5, organic silica cells in the form of fibroblasts, indicating possible effects on bone mass, since it consists largely of this protein. The results suggest beneficial effects, with the highest dose increasing the concentration of collagen. Secondly, treatment with Loïc Le Ribault'sG5, organic silica osteoblasts in process of differentiation results in an increase in calcification compared to untreated cells, as indicated by an increased calcium deposition, finding Loïc Le Ribault'sG5, organic silica one of the products capable of increasing mineralization of culture osteoblasts.

 

There could be two reasons for the observed increase of the calcium deposit located in the osteoblasts. Firstly, the greater differentiation of fibroblasts, induced by treatment resulting in an increased number of osteoblasts and hence proportionally increasing calcification. Secondly an increase of calcium in the metabolism or cellular processes involved in the deposition of calcium would explain increased calcification.

 

In mature osteoblasts, treatment with Loïc Le Ribault'sG5, organic silica results in a significant increase in alkaline phosphatase activity. Since this activity depends on the concentration of collagen in the extracellular matrix and is at the same time responsible for the calcification. The increased collagen synthesis induced  by treatment using Loïc Le Ribault'sG5, organic silica  was responsible for the increased activity of the alkaline phosphatase, noting that the determination of collagen and alkaline phosphatase, has been achieved in two different experiments, therefor  the increase in alkaline phosphatase and calcification  in the cells treated with Loïc Le Ribault'sG5, organic silica  is due to the higher concentration of collagen  in the extracellular matrix, an effect  magnified and clearly significant when it comes to growing fibroblasts.

 

The results of this study indicate that treatment with Loïc Le Ribault'sG5, organic silica increases collagen synthesis and reinforces the extracellular matrix in MG-63 cells. This effect potentiates calcium deposition by osteoblasts increasing calcification of the extracellular matrix. If these results were passed on to humans, the use of SILICIUM G5 would be beneficial in situations in which a reinforcement of bone matrix in the context of an adequate intake of necessary components required to induce the differentiation from fibroblasts to osteoblasts or osteogenesis.

 

Centro Tecnológico de Nutrición y Salud

Dr. José María del Bas, Dr. Luis Arola y Dr. Francesc Puiggròs

Reus, July 2014

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