Silica Effects on Collagen Production

IEC Espagne

Institute of Spanish Clinical Expertise 

Dosification of Collagen Neo-Synthesis

Study of product effects on the production of collagen neo-synthesis in human fibroblasts.

Report:  In Vitro Clinical Study

At the request of the INSTITUT D’EXPERTISE CLINQUE – ESPAGNE, we have evaluated the effect of the Loïc Le Ribault's G5 organic silica in the following parameters:

  • Synthesis of Collagen in Human Fibroblasts
  • Cytoxicity in Human Fibroblasts

What is collagen?

Collagen is the most abundant protein and is naturally produced by the body. The production of this substance diminishes as people grow older. It is significant in making certain parts of the body and is also an important factor in joint health, bones, cartilage, tendons, ligamentsmuscle tissuecorneas, blood vessels, fascia, gut, intervertebral discs, teeth & gums. It is also important on the moisture, smoothness, elasticity and flexibility of the skin, hair & nails.


Evaluation of Cytoxicity

The results are presented in the following table:



Optical density





Negative Test

0.512 ± 0.03


Organic Silica (0.2%)

0.508 ± 0.04


Organic Silica (0.5%)

0.500 ± 0.06


Organic Silica (1%)

0.514 ± 0.04


 NS: Not Significant  


The results show that the Loïc Le Ribault's G5 organic silica does not present cytoxicity with regard to any human fibroblasts in culture, when  used to 0.2%, 0.5% and 1.%.


Evaluation of the Collagen Index

The separation and identification of hydroxyproline were performed by reversed-phase HPLC. Fluorescence peaks, after integration, used to calculate the concentration of hydroxyproline in the medical culture.


The results are presented in the following table: 


Concentration of 



  hydroxyproline (mg/ml)

% increase

Negative Test

1.64 ± 0.10


Organic Silica G5 0.2%

1.79 ± 0.13


Organic Silica G5 0.5%

1.86 ± 0.08


Organic Silica G5 1%

1.95 ± 0.10



The results show that Loïc Le Ribault's G5 Organic Silica significantly increments the level of collagen produced by the human fibroblast cultures by comparison with the control.



In the experimental conditions adopted for the product Organic Silica G5:

  • Induces a significant increase in collagen levels of 13% and 19% respectively for the concentrations of 0.5% and 1% in cultured human fibroblasts compared with the control.  
  • Does not induce cytoxicity in the concentrations (0.2%, 0.5%, and 1%)

In conclusion, the Organic Silica G5 product induces a significant increase in the index in cultured human fibroblasts.


Product being investigated:                      Organic Silica (ref: Silicium G5)

Spanish I.E.C. Code:                                   E080043 130003 

Experiment Protocol:                                  No. E080043PE, of February 26, 2008



Study No. E080043RD


Study Sponsor                                                                                       Study Manager

Mr. J.C. Hierro                                                                                      Mr. J. C. Alonso

Director General                                                                                   Master in Microbiology        

Silicium Espana                                                                                    I.E.C. Spain

Cerviago 5                                                                                             C/Caspe, 104, Bajos

33875 Tineo                                                                                           08010 Barcelona




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